Life Cell Imaging
Fluorescence Lifetime Imaging
Super-resolution Microscopy
homoFRET / heteroFRET
Expression and Labeling of Proteins
Lipid Nanodiscs (SMALPs)
Fluorescence Proteins
FCS
Antibrownian Electrokinetic Trap (ABELtrap)
Fluorescence Lifetime Spectroscopy
Hello! I’m Lukas Spantzel. I am passionate about photonics and biotechnnology. I am currently working with single-molecule micorscopy to investigate G-Protein couples receptors, mainly neurotensin receptor 1.
In my past work I acquired a wide range of skills, from membrane protein purification to superresolution microscopy. I am driven by my boundless enthusiasm and thirst for knowledge.
| Age | 30 |
| post@lukasspantzel.de | |
| Location | Jena, Germany |
Structure of lymphostatin, a large multi-functional virulence factor of pathogenic Escherichia coli.
M. Griessmann, T. Rasmussen, V.J. Flegler, C. Kraft, R. Schneider, M. Hateley, L. Spantzel, M.P. Stevens, M. Börsch, and B. Böttcher (2025). In: Nat Commun 16, 5389. https://doi.org/10.1038/s41467-025-60995-9
Lymphostatin: Structure of a large multi-functional virulence factor
M. Griessmann, T. Rasmussen, V. J. Flegler, C. Kraft, R. Schneider, L. Spantzel, M. P. Stevens, M. Börsch, B. Böttcher (2024). bioRxiv 2024.05.21.595110; https://doi.org/10.1101/2024.05.21.595110
Multichannel-GaAsP-photomultiplier-based fiber bundle ISM-STED microscope
M. Babin, S. Fabian, M. Reibe, L. Spantzel, M. Börsch, and E. Beckert (2024). In: Proceedings Volume 13025, Advanced Photon Counting Techniques XVIII; 1302507. https://doi.org/10.1117/12.3012731
Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infrared photoinduced force microscopy (PiF-IR)
J. Joseph, L. Spantzel, M. Ali, D. Moonnukandathil Joseph, S. Unger, K. Reglinski, C. Krafft, A.-D. Müller, C. Eggeling, R. Heintzmann, M. Börsch, A. T. Press, and D. Täuber (2023). In: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, S. 123612. https://doi.org/10.1016/j.saa.2023.123612
A BODIPY‐Based Molecular Rotor in Giant Unilamellar Vesicles: A Case Study by Polarization‐Resolved Time‐Resolved Emission and Transient Absorption Spectroscopy
K. K. Jha, A. Prabhakaran, L. Spantzel, R. C. Sia, I. Pérez, R. A. Arellano‐Reyes, A. Elmanova, A. Dasgupta, C. Eggeling, M. Börsch, J. Guthmuller, M. Presselt, T. E. Keyes, and B. Dietzek-Ivanšić (2023). In: ChemPhotoChem, Artikel e202300091. https://doi.org/10.1002/cptc.202300091
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs
L. Spantzel, I. Pérez, T. Heitkamp, A. Westphal, S. Reuter, R. Mrowka, and M. Börsch (2023). In: Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 1238407. https://doi.org/10.1117/12.2648222
Trends in Microscopy – 3rd German Bioimaging Spring School (2025)
Hands-On Workshop: Monitoring activation of the GPCR NTSR1 in living cells by FLIM-heteroFRET and homoFRET
German Bioimaging FLIM Workshop (2024)
Hands-On Workshop: FLIM-FRET with fluorescent proteins
International SMALP Conference (2023)
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs
XXIII. Linz Winter Workshop (2024)
Single-molecule spectroscopy of GPCR oligomerization using a Anti-Brownian Electrokinetic Trap (ABELtrap)
Meeting of the Signal Transduction Society (2023)
Single-molecule spectroscopy of human neurotensin receptors 1 in single lipid nanodiscs and extracellular vesicles for monitoring ligand-induced oligomerization
25th Jubilee Meeting on Signal Transduction (2022)
Monitoring ligand-induced oligomerization of human neurotensin receptors 1 in living cells and trapped in single lipid nanodiscs by homoFRET microscopy
10th doctoral conference on optics & photonics (DoKDoK 2022):
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells by time-resolved homoFRET-STED microscopy
Student poster competition at University of Applied Sciences Jena (2018)
Optimization and characterization of a fluorometric two-component sensor for the detection of saccharides (2nd Prize)
since 2021
PhD candidate
at Single-Molecule Microscopy Group, Jena University Hospital
Applying different methods of super-resolution microscopy, like FLIM and anisotropy imaging, STED, SIM/STROM. Accompanied by a range of methods from biochemistry, molecular biology and cell culture. Preparation of PhD thesis on fluorescence spectroscopic single molecule studies of the neurotensin receptor 1.
2020 - 2021
Master thesis
at Synlab Jena Oncoscreen
Assay design of LAMP (loop-mediated isothermal amplification) and RPA (replicase polymerase amplification) as an alternative to PCR based on commercial kits. Verification of success with Sanger sequencing and qPCR.
2020 - 2021
Lecturer
at University of Applied Sciences Jena
Gave the online practical course "Process Analytics" in the bachelor's degree program in biotechnology. Focus: UV/VIS and fluorescence spectroscopy
2020
Laboratory Assistant
at Synlab Jena Oncoscreen
Conducting corona PCR assays, including laboratory data management, RNA isolation, qPCR, and evaluation. Both manual and semi-automated including workflow optimization.
2019 - 2020
IT Manager
at Students council, University of Applied Sciences Jena
Responsibility for the IT infrastructure of the student council, user support and advising the body on technical issues. Secondary job in addition to honorary position in the student council.
2018
Bachelor thesis
at Single-Molecule Microscopy Group, University Hospital Jena
Investigation of the GPCR neurotensin receptor after ligand binding using anisotropy and FRET measurements. Development of a method to attach two fluorescent dyes to a protein for the purpose of active-state distance measurement in single-molecule microscopy (confocal Förster resonance transfer microscopy). Construction, expression, purification, and reconstitution of membrane-bound fusion proteins.
2017
Student research project
at University of Applied Sciences Jena
Selection and optimization of an optical sensor system for the detection of glucose and other saccharides using fluorimetric measurement methods in complex media for monitoring glucose concentration in cell culture.
2016
Internship
at Single-Molecule Microscopy Group, University Hospital Jena
Preparation of the membrane protein neurotensin receptor 1
since 2021
Doctoral student
at Faculty of Medicine, Jena University
Degree pursued: Dr. rer. nat. in Biophysics.
2018 - 2021
Studies in pharmaceutical biotechnology
at University of Applied Sciences Jena
Degree: Master of Science
2015 - 2018
Studies in biotechnology
at University of Applied Sciences Jena
Degree: Bachelor of Engeneering
2013 - 2015
Studies in Biology
at Jena University
