Lukas Spantzel
PhD candidate in biophysics
About Me
Hello! I’m Lukas Spantzel. I am passionate about photonics and biotechnnology. I am currently researching single-molecule micorscopy to investigate G-Protein couples receptors, mainly neurotensin receptor 1.
In my past work I acquired a wide range of skills, from membrane protein purification to superresolution microscopy. I'm driven by the motivation to better understand the world around us.
Bio
Age
29
Email
post@lukasspantzel.de
Location
Jena, Thuringia, Germany
Professional Skills
Super-resolution microscopy
Protein Purification
Cell culture
Molecular Biology
Life cell imaging
Single-molecule spectroscopy
Programming for scientific purposes
Statistics
Publications
Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infrared photoinduced force microscopy (PiF-IR)
J. Joseph, L. Spantzel , M. Ali, D. Moonnukandathil Joseph, S. Unger, K. Reglinski, C. Krafft, A.-D. Müller, C. Eggeling, R. Heintzmann, M. Börsch, A. T. Press, and D. Täuber(2023). In: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, S. 123612. DOI: 10.1016/j.saa.2023.123612.
A BODIPY‐Based Molecular Rotor in Giant Unilamellar Vesicles: A Case Study by Polarization‐Resolved Time‐Resolved Emission and Transient Absorption Spectroscopy
K. K. Jha, A. Prabhakaran, L. Spantzel, R. C. Sia, I. Pérez, R. A. Arellano‐Reyes, A. Elmanova, A. Dasgupta, C. Eggeling, M. Börsch, J. Guthmuller, M. Presselt, T. E. Keyes, and B. Dietzek-Ivanšić (2023). In: ChemPhotoChem, Artikel e202300091, e202300091. DOI: 10.1002/cptc.202300091.
Monitoring oligomerization dynamics of individual human neurotensin receptors 1 in living cells and in SMALP nanodiscs.
L. Spantzel, I. Pérez, T. Heitkamp, A. Westphal, S. Reuter, R. Mrowka, and M. Börsch(2023). In: Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 1238407. DOI: 10.1117/12.2648222
Work Experience
PhD candidate at Single-Molecule Microscopy Group, Jena University Hospital
Aug 2021 - Present
Applying different methods of super-resolution microscopy, like FLIM and anisotropy imaging, STED, SIM/STROM. Accompanied by a range of methods from biochemistry, molecular biology and cell culture. Preparation of PhD thesis on fluorescence spectroscopic single molecule studies of the neurotensin receptor 1.
Master thesis at Synlab Jena Oncoscreen
Aug, 2020 - May, 2021
Assay design of LAMP (loop-mediated isothermal amplification) and RPA (replicase polymerase amplification) as an alternative to PCR based on commercial kits. Verification of success with Sanger sequencing and qPCR.
Lecturer at University of Applied Sciences Jena
Oct, 2020 - Feb, 2022
Conducting the online practical course "Process Analytics" in the bachelor's degree program in biotechnology. Focus: UV/VIS and fluorescence spectroscopy
Laboratory Assistant at Synlab Jena Oncoscreen
May, 2020 - Nov, 2020
Conducting corona PCR assays, including laboratory data management, RNA isolation, PCR, and evaluation. Both manual and semi-automated including workflow optimization.
IT Manager at Students council, University of Applied Sciences Jena
Feb, 2019 - Jan, 2020
LResponsibility for the IT infrastructure of the student council, user support and advising the body on technical issues. Secondary job in addition to honorary position in the student council.
Bachelor thesis at Single-Molecule Microscopy Group, University Hospital Jena
Mar, 2018 - Aug, 2018
Investigation of the GPCR neurotensin receptor after ligand binding using anisotropy and FRET measurements. Development of a method to attach two fluorescent dyes to a protein for the purpose of active-state distance measurement in single-molecule microscopy (confocal Förster resonance transfer microscopy). Construction, expression, purification, and reconstitution of membrane-bound fusion proteins.
Student research project at University of Applied Sciences Jena
Aug, 2017 - Nov, 2017
Selection and optimization of an optical sensor system for the detection of glucose and other saccharides using fluorimetric measurement methods in complex media for monitoring glucose concentration in cell culture.
Internship at Single-Molecule Microscopy Group, University Hospital Jena
Aug, 2016 - Sep, 2016
Preparation of the membrane protein neurotensin receptor 1.
Education
Doctoral student at the Faculty of Medicine at Friedrich-Schiller-University Jena
2021 - Present
Degree pursued: Dr. rer. nat. in biophysics.
Studies in pharmaceutical biotechnology at University of Applied Sciences Jena
2018 - 2021
Degree: Master of Science
Studies in biotechnology at University of Applied Sciences Jena
2015 - 2018
Degree: Bachelor of Engeneering.
Studies in Biology Friedrich-Schiller-University Jena
2013 - 2015